These platforms available include: Illumina’s MiSeq, HiSeq2500 and HiSeq 4000, Ion Proton and PacBio RSII. The HTSF is a full-service sequencing facility that offers multiple platforms and several different state-of-the-art techniques to assist with genetic and genomic research. High throughput sequencing is an extremely fast developing technology, used in many applications such as genome sequencing, genome re-sequencing, chip-seq, cDNA sequencing, digital expression, and microRNA abundance. The High-Throughput Sequencing Facility applies the tools of high throughput sequencing technology to enrich cancer research of UNC scientists. Sequencing High-Throughput Sequencing Facility These studies include both FDA-approved government and industry-sponsored research. The Immune Monitoring and Genomics Facility (IMGF) provides investigators with cellular immunology, molecular immunology, and immunogenomics services that can delineate interactions between the immune system and tumor tissues. The IMGF focuses on clinical trial correlative research studies but supports basic research studies as well. This is a fast and effective way to amplify samples with RT-qPCR. A Tecan Freedom Evo © is used to set up reactions in a 384-well plate, which is then run by the Roche Lightcycler 480 II ©. The Vironomics Core uses several automated robotics for the large-scale real-time PCR. The unique automated robotics also enables the Vironomics Core to offer custom and commercial real-time PCR assays to clients. The Vironomics Core currently provides several real-time qPCR arrays for viruses and miRNAs profiling including: KSHV, EBV, HSV1 and HSV2, RRV, HCMV, NFkappaB, Pre-miRNA, and P53response12. This is accomplished using Ion Torrent S5, companion Ion Torrent Chef, and robot-assisted PCR in 96 and 384 well format. The Vironomics Core facilitates research at UNC by performing large-scale real time quantitative PCR reactions, amplicon generation, and ~200-600bp next generation sequencing. This equipment includes 2 Fluidics Station 450s and the GeneChip® Hybidization Oven 645. It has the ability to scan all research arrays manufactured by Affymetrix including the high density expression arrays which place an entire genome on one GeneChip®. Also, we have the Affymetrix GeneChip® Scanner 3000 7G Plus Targeted Genotyping System in the Core. We currently have Beckman Coulter’s Biomek® FXP Target Prep Express robot and the GeneTitan Instrument from Affymetrix which allow the processing of hundreds of microarray samples in a week, and processing of high throughput expression peg arrays and Axiom genotyping high throughput peg arrays. Functional Genomics Coreįunctional Genomics Core performs expression profiling and SNP genotyping (Affymetrix) services. The AA Core also offers assistance with end-user data analysis. Users receive their results electronically after work has been completed and can track the progress of their projects via cloud-based reports. Each project begins with an initial consultation to discuss experimental design and feasibility. The AA Core offers turn-key research solutions – bring us samples, we send you processed data. The CGIBD Advanced Analytics (AA) Core provides bulk and single-cell genomic and proteomic analysis, high-throughput qPCR, and cost-effective immunoassays. Expression Analysis Center for Gastrointestinal Biology and Disease (CGIBD): Advanced Analytics Core Mammalian Genotyping Core provides services using any of the Illumina microarrays (BeadChips) for genotyping, CNV analysis, or DNA methylation. Functional Genomics Core performs expression profiling and SNP genotyping (Affymetrix) services.
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